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 หัวเรื่อง:การศึกษาเชื้อไวรัสในปลาน้ำจืดที่เกิดโรคระบาดด้วยกล้องจุลทรรศน์อิเล็คตรอน ผู้เขียน: ดร.ณรงค์ จึงสมานญาติ, รองศาสตราจารย์ , นายกิติ ศรีสุภาพ, รองศาสตราจารย์,  Churee Pankamnerd, นายบุญธรรม จงเจริญ, รองศาสตราจารย์, Ong-ard Lawhavinij, Chantana Kunjara สื่อสิ่งพิมพ์:pdf Abstract Eighteen fresh water fish affected with ulcerative skin disease outbreak in years 1983-1984 from the fish farms and natural canals in Supanburi and Nakhon Pathom provinces were examined for virus particle by using transmission electron microscope. There was no virus particle observed in the ulcerative tissue from eight fish collected, a few different types of virus particles, however, were found in the live cells and one type in the splenic cells. These viral particles may be grouped into two groups according to their different sizes. The first group was a large spherical particles of 45-90 nm. having capsid surrounding the nucleic acid core. The other group was a small virus particles of 10-30 nm. with nonenveloped spherical or icosahedral shaped particles and were crystallized arrays of irregular arrays in cytoplasm or cytoplasmic membrane-bound inclusion body. The groups of virus particles were revealed much morphological similar to Reoviruses in the former and Picornaviruses in the later which are both RNA viruses. This study could not concluded that the virus particles observed were the causative agent of the ulcerative skin disease outbreak of fresh water fish. Further study on their significance in such disease outbreak in being conducted. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Characterization of Glycoconjugates Present in Cloacal Epithelium of the Chicken) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์ , นายบุญธรรม จงเจริญ, รองศาสตราจารย์, Suppaluk Romrattanapun สื่อสิ่งพิมพ์:pdf AbstractThe distribution of glycoconjugates in the chicken cloaca was studied by means of light microscopic histochemical methods. The staining procedures employed were horseradish peroxidase conjugated lectin, Alcian blue (AB) pH 1.0, AB pH 2.5, periodic acid –Schiff (PAS), AB pH 2.5-PAS in combination with enzyme neuraminidase digestion. The lectin used in the present study were peanut agglutinin (PNA), wheat germ agglutinin (WGA), Dolichos biflorus agglutinin (DBA), and Lotus tetragonolobus agglutinin (LTA) The mucosa of both coprodeum and urodeum is covered by simple columnar epithelium in which 2 type of cells are encountered-columnar and goblet cells. In contrast, one layer of only columna cells is found in the mucosa of proctodeum. Tubular glands of proctodeum are commonly provided at submucosal layer. All the goblet cells in coprodeum and urodeum and tubular glands of proctodeum were coloured blue and purple with AB pH 1.0 and AB pH 2.5-PAS, respectively. They were furthermore stained moderately with WGA. Neuraminidase digestion changed the blue coloration with AB pH 2.5 to pale blue. Removal of sialic acid with neuraminidase imparted weak to strong affinity of PNA. Histophysiological significances of glycoconjugates involved in the chicken cloaca were discussed with special reference to the functional activities of the carbohydrates. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Detection of Glycoconjuagates in Chicken Colonic Epithelium with a Specific Horseradish Peroxidase Conjuagated Lectin) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, นายบุญธรรม จงเจริญ, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractChicken colons were stained with a battery of seven horseradish peroxidase-conjugated lectins : Ricnus communis agglutinin-I (RCA-I) peanut agglutinin, wheat germ agglutinin, Ulex europaeus agglutinin, Limax fiavus agglutinin, Dolichos biflorus agglutinin, and Pisum sativum agglutinin. Lectin staining revealed striking differences in the structure of oligosaccharides between intracellular secretory glycoconjugates in goblet cells and in columnar cells. The mucous granules of goblet cells were found to contain glycoconjugates with sialic acid-galactose dimer,D-mannose,D-glucose, N-acety-D-glucosamine and D-galactose residues. In contrast, terminal or internal N-acety-D-glucosamine and terminal galactose-N (1-3)-acety-D-galactosamine disaccharides were detected in supranuclear region of the columner cells. The striated border of the colonic columnar cells were also found to contain glycoconjugates similar in reactivity to those of the goblet cells. The existence of glycoconjugates with various saccharide residues in colonic epithelium of the chicken is reported for the first time in this study. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : The Arteriovenous Connection in the Spermatic Cord in the Variable Squirrel (Callosciurus finlaysoni) ) ผู้เขียน:ดร.วรวุฒิ ฤกษ์อำนวยโชค, รองศาสตราจารย์ , ดร.ณรงค์ จึงสมานญาติ, รองศาสตราจารย์, ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, นายบุญธรรม จงเจริญ, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractIn the variable squirrel (Callosciurus finlaysoni), four to five ramifying arterioles arose directly from the testicular artery and then gave off numerous small capillaries. The capillaries made a series of anastomoses with neighbouring counterpart capillaries to become a complicated network. Some of the capillaries drained into a small venule, which were connected directly with the testicular vein (pampiniform plexus), to form an arteriovenous connection (A-V shunt) between the testicular artery and the pampiniform plexus. This A-V shunt appeared to make the transfer of substances from the pampiniform plexus to the testicular artery more efficient. The shunt might control the volume of the blood draining into the testis. In addition, the capillaries were covered by vesiculated cells which were located adjacent to the pericytes of the capillaries. The vesiculated cells contained abundant mitochondria, rough endoplasmic reticulum, Golgi complex and cytoplasmic vesicles. The vesiculated cells could provide support for the capillaries and prevented their collapse when the shunts were closed. |
 Researcherดร. ณรงค์ จึงสมานญาติ, รองศาสตราจารย์ที่ทำงาน:สำนักงานเลขานุการ คณะสัตวแพทยศาสตร์ สาขาที่สนใจ:มหกายวิภาคศาสตร์และจุลกายวิภาคศาสตร์ของสัตว์เลี้ยง, การวิภาคศาสตร์ประยุกต์ทางสัตวแพทย์ของม้า สัตว์เคี้ยวเอื้อง สุกร สุนัข แมวและไก่, การทดสอบฤทธิ์ฆ่าเห็บโค สุนัข Resume |